Natural Killer Cell Protocols : Cellular and Molecular Methods (Methods in Molecular Biology, Vol 121)
|von Kerry S. Campbell|
From Book News, Inc. A collection of readily reproducible methods designed to study natural killer cells (a subset of lymphocytes that can directly lyse some tumor cells and certain virally affected cells without prior sensitization). Campbell (Fox Chase Cancer Center) and Colonna (Basel Institute for Immunology) present classic techniques and more recent innovations. Techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, and calcium mobilization in cell conjugates.Book News, Inc.®, Portland, OR
Synopsis This text presents a comprehensive collection of detailed experimental protocols for studying the cell biology, biochemistry, and molecular biology of natural killer (NK) cells. These cutting-edge techniques include methods for cloning and culturing, measuring cytoxicity against tumours and virally infected cells, analysis of development in vitro, migration assays, signal transduction testing, detecting cytokine production, and protein expression approaches. There are also methods for applying...
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