In Situ Hybridization Protocols (Methods in Molecular Biology, 123)
Book News, Inc. Darby (human biology, RMIT U., Victoria, Australia) is joined by geneticists, molecular biologists, and pathologists from around the world to describe basic and advanced techniques for hybridization, for whole-mount embryo specimens and at the electron microscope level. Coverage includes protocols for detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA and RNA probes to detect expression in cells or tissue sections. For novice and experienced investigators who need proven and readily reproducible methods. -- Copyright © 1999 Book News, Inc., Portland, OR All rights reserved Book News, Inc.®, Portland, OR
Synopsis Updated for the second edition, this collection of state-of-the-art basic and advanced techniques for in situ hybridization aims to assist researchers in attempting this difficult technique in their own laboratories and, for those already doing it, to update and improve their methods. Each protocol provides detailed easy-to-follow instructions along with realistic commentaries on applications and troubleshooting.
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