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NachrichtenLexikonProtokolleBücherForenDonnerstag, 21. August 2014 

Capillary Electrophoresis of Nucleic Acids, Vol. II: Practical Applications of Capillary Electrophoresis


von Keith R. Mitchelson

Kategorie: Molekularbiologie
ISBN: 0896037657

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Synopsis Capillary electrophoresis (CE) provides researchers with the ability to separate and analyze very small amounts of DNA. CE offers important advantages over other available methods because of the speed and reproducibility of analysis. Moreover, the high sensitivity of detection and the ability to increase sample throughput with parallel analysis has led to the creation of a full range of techniques for the analysis of DNA molecules, from modified DNA-adducts and single strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. "Capillary Electrophoresis of Nucleic Acids" provides these analytical protocols, which can be used for detection and analysis of mutations and modifications to precise DNA loci through to entire genomes of organisms. Volume II of this two-volume set broadly addresses techniques for high-throughput analysis of DNA fragments of less than 1 kbm employing SNP detection, mutation detection, DNA sequencing methods, and DNA ligand interactions. It presents practical considerations necessary for very rapid and accurate separations of linear DNAs by CE using short capillaries. Also covered are the various modes of genotyping by CE with an emphasis on the use of multiplex separations followed by techniques for the quantitative estimation of gene suppression using CE.


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