Two-Hybrid Systems : Methods and Protocols (Methods in Molecular Biology Vol. 177.)

von Paul N. MacDonald Kategorie: Molekularbiologie ISBN: 0896038084 Kommentar abgeben

Synopsis Designed to appeal to a broad range of scientists using two-hybrid systems in experimental biology, this book provides the basic methodologies for working with the yeast two-hybrid system. It then covers the topic of "artifacts" (false positives) that are inherent in the system and offers rational strategies to avoid them. The expert authors also discuss a variety of specific systems in which the two-hybrid system has had the greatest impact. The final section of the book describes variations on the traditional two-hybrid system that have been used effectively in scientific research.