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Windows into the Cells Interior - New Method Enables Deeper Insights into the Cell

19.03.2012 - (idw) Max-Planck-Institut für Biochemie

Cryo-electron tomography provides high-resolution, three-dimensional insights into the cell. However, with this method only very small cells or thin peripheral regions of larger cells can be investigated directly. Scientists of the Max Planck Institute of Biochemistry (MPIB) in Martinsried near Munich have now developed a procedure to provide access to cellular regions which were previously nearly inaccessible. Using focused ion beam (FIB) technology, specific cellular material can be cut out, opening up thin windows into the cells interior. This alternative approach enables the preparation of larger cellular samples devoid of artefacts. The study was recently published in PNAS USA. With cryo-electron tomography, pioneered by the Department of Molecular Structural Biology headed by Wolfgang Baumeister, researchers can now directly analyze three-dimensional cellular structures. The entire cell or individual cell components are shock frozen and enclosed in glass-like ice, thus preserving their spatial structure. The transmission electron microscope then enables the acquisition of two-dimensional projections from different perspectives. Finally, the scientists reconstruct a high-resolution three-dimensional volume from these images. However, the electron beam can penetrate only very thin specimens (for example bacteria cells) up to a thickness of 500 nanometers. Cells of higher organisms are clearly thicker. State-of-the-art electron microscopic preparation techniques are therefore necessary to make also larger objects accessible for cryo-electron tomography. The artefact-free and, in particular, targeted preparation of larger cells is a critical step, explained Alexander Rigort, MPIB scientist. With the traditional methods, we could never rule out that structures we wanted to investigate were changed. The meaningfulness of the results was therefore limited, according to the biologist.

Using a focused ion beam microscope (FIB), researchers can now mill single layers of the frozen-hydrated cell and remove them in a controlled manner thus rendering thin, tailor-made electron-transparent windows. An additional advantage of ion thinning is that mechanical sectioning artefacts are completely avoided. This method was originally developed for the material sciences. In structural biology the method shall now provide deeper insights into the molecular organization of the cells interior. The thinner the windows are, the higher the attainable resolution in the electron microscope. Now precise insights into the macromolecular architecture of cell regions are possible that were previously nearly inaccessible for cryo-electron microscopy, said Jürgen Plitzko, scientist at the MPIB.

Original Publication
A. Rigort, F. Bäuerlein, E. Villa, M. Eibauer, T. Laugks, W. Baumeister and J. M. Plitzko: Focused Ion Beam micromachining of eukaryotic cells for cryoelectron tomography. Proc. Natl. Acad. Sci. USA, March 5, 2012
Doi:10.1073/pnas.1201333109.

Contact
Dr. Jürgen M. Plitzko
Molecular Structural Biology
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
E-Mail: plitzko@biochem.mpg.de
http://www.biochem.mpg.de/baumeister

Dr. Alexander Rigort
Molecular Structural Biology
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
E-Mail: rigort@biochem.mpg.de
http://www.biochem.mpg.de/baumeister

Anja Konschak
Public Relations
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
Phone: +49 (0) 89 8578-2824
E-Mail: konschak@biochem.mpg.de
http://www.biochem.mpg.de jQuery(document).ready(function($) { $("fb_share").attr("share_url") = encodeURIComponent(window.location); });

Weitere Informationen: http://www.biochem.mpg.de/en/news/pressroom/index.html - Press releases of the Max Planck Institute of Biochemistry http://www.biochem.mpg.de/baumeister - Website of the Research Department Molecular Structural Biology (Wolfgang Baumeister)
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